{"product_id":"bek1343","title":"Rat B7-2\/CD86 ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of B7-2 \/ CD86 in rat serum, plasma, body fluids, tissue lysatesor cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Cluster of Differentiation 86 (also known as CD86 and B7-2) is a protein constitutively expressed on dendritic cells, Langerhans cells, macrophages, B-cells (including memory B-cells), and on other antigen-presenting cells.Along with CD80, CD86 provides costimulatory signals necessary for T cell activation and survival. Depending on the ligand bound, CD86 can signal for self-regulation and cell-cell association, or for attenuation of regulation and cell-cell disassociation.The CD86 gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily.Alternative splicing results in two transcript variants encoding different isoforms. Additional transcript variants have been described, but their full-length sequences have not been determined.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-B7-2 \/ CD86 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-B7-2 \/ CD86 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to theB7-2 \/ CD86amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofB7-2 \/ CD86can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 62.5pg\/ml - 4000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt;10 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409053552920,"sku":"BEK1343","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_6ba40957-dfcc-452d-8b8c-00d8484b2fce.jpg?v=1750988954","url":"https:\/\/danabiosci.com\/products\/bek1343","provider":"Dana Bioscience","version":"1.0","type":"link"}