{"product_id":"bek1339","title":"Mouse BCMA\/TNFRSF17 ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of BCMA \/ TNFRSF17 in mouse serum, plasma, body fluids, tissue lysatesor cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).Serum B-cell maturation antigen (sBCMA) is the cleaved form of BCMA, found at low levels in the serum of normal patients and generally elevated in patients with multiple myeloma (MM).\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-BCMA \/ TNFRSF17 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-BCMA \/ TNFRSF17 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to theBCMA \/ TNFRSF17amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofBCMA \/ TNFRSF17can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 31.2 pg\/ml - 2000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt;7 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409053126936,"sku":"BEK1339","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_db42783f-c222-45ce-ac1d-65fbdc6c46cf.jpg?v=1750988945","url":"https:\/\/danabiosci.com\/products\/bek1339","provider":"Dana Bioscience","version":"1.0","type":"link"}