{"product_id":"bek1328","title":"Human EpCAM \/ CD326 ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of EpCAM \/ CD326 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Epithelial cell adhesion molecule (EpCAM), also known as CD326 among other names, is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell–cell adhesion in epithelia.EpCAM is also involved in cell signaling,migration,proliferation, and differentiation.Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A \u0026amp; E.Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-EpCAM \/ CD326 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-EpCAM \/ CD326 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the EpCAM \/ CD326 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of EpCAM \/ CD326 can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 15.6 pg\/ml - 1000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 7.8 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409050013976,"sku":"BEK1328","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_2b39cfd7-12a5-45b2-aad7-dc1a13168008.jpg?v=1750988920","url":"https:\/\/danabiosci.com\/products\/bek1328","provider":"Dana Bioscience","version":"1.0","type":"link"}