{"product_id":"bek1291","title":"Human AMICA \/ JAML ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of AMICA \/ JAML in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e JAML or Junctional Adhesion Molecule-Like, or AMICA1 is a JAM transmembrane protein family member.It is composed of two extracellular immunoglobulin-like domains, a membrane-spanning region, and a cytoplasmic tail involved in activation signaling. A known ligand of JAML is Coxsackie virus and Adenovirus Receptor (CXADR in human s and CAR in mice) which has been shown to localize to the tight junctions of epithelial cells.JAML-mediated activation of CAR is required for neutrophil extravasationin addition to other leukocyte\/epithelial cell interaction models.Other members of the JAM family of transmembrane proteins include JAM1, JAM2 and JAM3.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-AMICA \/ JAML polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-AMICA \/ JAML polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the AMICA \/ JAML amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of AMICA \/ JAML can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 62.5 pg\/ml - 4000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 10 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409045852440,"sku":"BEK1291","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_c0e1d7a8-5274-447e-96b6-fabacd1c37be.jpg?v=1750988845","url":"https:\/\/danabiosci.com\/products\/bek1291","provider":"Dana Bioscience","version":"1.0","type":"link"}