{"product_id":"bek1287","title":"Human IL-23 ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of IL-23 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Interleukin-23 subunit alpha(IL-23) is a 189-amino acid protein that in human s is encoded by the IL23A gene. It is composed of the IL12 p40 subunit and the p19 subunit (IL23A), and is produced by dendritic cells and macrophages. Moreover, IL-23 is stimulated by Danger Signals, including cell debris, and directs memory T cells to Th17 response. It is an important part of the inflammatory response against infection. Recently, IL-23 has been implicated in the development of cancerous tumors. Langowski et al. (2006) showed that IL23 is an important molecular link between tumor-promoter proinflammatory processes and the failure of the adaptive immune surveillance to infiltrate tumors.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-23 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-23 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-23 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-23 can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 156 pg\/ml - 10,000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 25 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409045295384,"sku":"BEK1287","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_2c4a265d-e597-4b9b-ab6b-1a00cbdc4d3d.jpg?v=1750988837","url":"https:\/\/danabiosci.com\/products\/bek1287","provider":"Dana Bioscience","version":"1.0","type":"link"}