{"product_id":"bek1210","title":"Human TIMP-2 ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of TIMP-2 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e TIMP metallopeptidase inhibitor 2, also known as TIMP-2, is a 21-kD protein, which is secreted by human melanoma cells and binds to type IV collagenase proenzyme secreted by the same cells. The TIMP2 gene is a member of the TIMP gene family, It is encoded by 5 exons spanning 83 kb of genomic DNA. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. It has an amino acid sequence with many similarities to human tissue inhibitor of metalloproteinase (TIMP1). TIMP2 is a tissue inhibitor of metalloproteinases, TIMP2 induced a decrease in total protein tyrosine phosphatase (PTP) activity associated with beta-1 integrin subunits as well as dissociation of the phosphatase SHP1 from beta-1.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-TIMP-2 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-TIMP-2 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TIMP-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TIMP-2 can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 156 pg\/ml-10,000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt;  2 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409038446872,"sku":"BEK1210","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_c0ab7738-0120-445c-9cd2-8a6cba673821.jpg?v=1750988695","url":"https:\/\/danabiosci.com\/products\/bek1210","provider":"Dana Bioscience","version":"1.0","type":"link"}