{"product_id":"bek1152","title":"Human MIP-1α ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of MIP-1α in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Macrophage inflammatory protein-1(MIP-1a), also known as Chemokine (C-C motif) ligand 3(CCL3) or LD78, is a cytokine belonging to the CC chemokine family. The LD78 gene chromosome 17q21.1-q21.3. It is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Sherry et al. (1988) demonstrated 2 protein components of MIP1, called by them alpha and beta. MIP-1a is an important mediator of virus-induced inflammation in vivo, and also an important second signal for mast cell degranulation in the conjunctiva and for acute-phase disease, possibly through interaction with CCR1, its chemokine receptor.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-MIP-1α polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-MIP-1α polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the MIP-1α amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of MIP-1α can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 15.6 pg\/ml - 1000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 10 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409028092184,"sku":"BEK1152","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_8a1ea0de-0961-4554-a752-7cdafd182330.jpg?v=1750988568","url":"https:\/\/danabiosci.com\/products\/bek1152","provider":"Dana Bioscience","version":"1.0","type":"link"}