{"product_id":"bek1126","title":"Rat IL-18 ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of IL-18 in rat serum, plasma, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Interleukin-18 (IL18), also known as interferon-gamma inducing factor, in human s is encoded by the IL18 gene, this gene contains 6 exons, and maps to 11q22.2-q22.3. It is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. It works by binding to the interleukin-18 receptor, and together with IL-12 it induces cell-mediated immunity following infection with microbial products like lipopolysaccharide (LPS). IL-18 has been implicated as an inflammatory mediator of Hashimoto's thyroiditis, the most common cause of autoimmune hypothyroidism. IL-18 is also able to induce severe inflammatory reactions, which suggests its role in certain inflammatory disorders.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- IL-18 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- IL-18 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-18 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-18 can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 15.6 pg\/ml - 1000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 1 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409025700120,"sku":"BEK1126","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_ca16ebf9-dfea-4725-be1c-f9aabadf7e9c.jpg?v=1750988508","url":"https:\/\/danabiosci.com\/products\/bek1126","provider":"Dana Bioscience","version":"1.0","type":"link"}