{"product_id":"bek1122","title":"Human IL-15 ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of IL-15 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Interleukin 15 (IL-15) is a cytokine that affects T-cell activation and proliferation similarly to IL2. It secreted by mononuclear phagocytes (and some other cells) following infection by virus(es) and normally presented in vivo as a cell-associated cytokine bound to IL15RA. IL-15 binds to and signals through a complex composed of IL-2\/IL-15 receptor beta chain (CD122) and the common gamma chain (gamma-C, CD132). In rodent lymphocytes, IL-15 prevents apoptosis by inducing an apoptosis inhibitor, BCL2L1\/BCL-x(L). In human s with celiac disease IL-15 similarly suppresses apoptosis in T-lymphocytes by inducing Bcl-2 and\/or Bcl-xL. There have been recent studies suggesting that suppression of IL-15 may be a potential treatment for celiac disease and even presents the possibility of preventing its development.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-15 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-15 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-15 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-15 can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 46.9 pg\/ml  - 3000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 3 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409025274136,"sku":"BEK1122","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_291f033a-7360-4124-b175-271e7c0c64c7.jpg?v=1750988498","url":"https:\/\/danabiosci.com\/products\/bek1122","provider":"Dana Bioscience","version":"1.0","type":"link"}