{"product_id":"bek1115","title":"Mouse IL-10 ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of IL-10 in mouse serum, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Interleukin-10 (IL-10 or IL10) also known as human cytokine synthesis inhibitory factor (CSIF), is an anti-inflammatory cytokine. In human s, IL-10 is encoded by the IL10 gene, which is located on chromosome 1 and comprises 5 exons. The IL-10 protein is a homodimer, each of its subunits is 178-amino-acid long. It is primarily produced by monocytes. IL-10 is a cytokine with pleiotropic effects in immunoregulation and inflammation. It could downregulates the expression of Th1 cytokines, MHC class II antigens, and co-stimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-10 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-10 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washedaway with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-10 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-10 can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 15.6 pg\/ml - 1000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 5 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409024651544,"sku":"BEK1115","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_17e67a83-5f78-4fdc-b78c-869aed5847ec.jpg?v=1750988481","url":"https:\/\/danabiosci.com\/products\/bek1115","provider":"Dana Bioscience","version":"1.0","type":"link"}