{"product_id":"bek1097","title":"Human IL-1RA ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of IL-1RA in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e The interleukin-1 receptor antagonist (IL-1RA) is a protein that in human s is encoded by the IL1RN gene which maps to 2q14.2. It is a member of the interleukin 1 cytokine family, and it is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, IL-1RA is an agent that binds non-productively to the cell surface interleukin-1 receptor (IL-1R). Carter et al. (1990) found that IL1RN specifically inhibited IL1 bioactivity on T cells and endothelial cells in vitro and was a potent inhibitor of IL1-induced corticosterone production in vivo. Mutations in the IL1RN gene results in a rare disease called deficiency of the interleukin-1–receptor antagonist (DIRA), and elevated levels of IL1RN has been found in serum of schizophrenia patients.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IL-1RA polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IL-1RA polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IL-1RA amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IL-1RA can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 31.2 pg\/ml - 2000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 2 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409022390552,"sku":"BEK1097","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_5fe51143-193f-484f-966f-17b95eeaf169.jpg?v=1750988438","url":"https:\/\/danabiosci.com\/products\/bek1097","provider":"Dana Bioscience","version":"1.0","type":"link"}