{"product_id":"bek1088","title":"Human IGFBP-1 ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of IGFBP-1 in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Insulin-like growth factor-binding protein 1 (IGFBP-1) also known as placental protein 12 (PP12) is a member of the Insulin-like growth factor-binding protein (IGFBP) family. It is synthesized in liver, secretory endometrium, and decidua. The IGFBP1 gene has 4 exons, spans 5.9 kb, and mapped to 7p13-p12. This protein has an IGFBP domain and a type-I thyroglobulin domain. It binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. Leu and George (2007) concluded that IGFBP1 is a negative regulator of the p53\/BAK-dependent pathway of apoptosis.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-IGFBP-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-IGFBP-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the IGFBP-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of IGFBP-1 can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 31.2 pg\/ml - 2000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 1 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409021276440,"sku":"BEK1088","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_69fa96cd-05a0-4198-9ab9-301c70cc90d0.jpg?v=1750988417","url":"https:\/\/danabiosci.com\/products\/bek1088","provider":"Dana Bioscience","version":"1.0","type":"link"}