{"product_id":"bek1072","title":"Mouse GM-CSF ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of GM-CSF in mouse serum, plasma, body fluids, tissue lysates or cell culture supernate.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a protein secreted by macrophages, T cells, mast cells, NK cells, endothelial cells and fibroblasts. It is essential for the in vitro proliferation and differentiation of precursor cells into mature granulocytes and macrophages. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is used as a medication to stimulate the production of white blood cells following chemotherapy. Recently, it has been evaluated in clinical trials for its potential as a vaccine adjuvant in HIV-infected patients. The preliminary results have been promising but GM-CSF is not presently FDA-approved for this purpose.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-GM-CSF polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GM-CSF polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrateswere used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the GM-CSF amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of GM-CSF can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 7.8 pg\/ml - 500 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 5.8 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409020326168,"sku":"BEK1072","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_8eaeaa54-b330-4438-b292-2a5c426c6097.jpg?v=1750988387","url":"https:\/\/danabiosci.com\/products\/bek1072","provider":"Dana Bioscience","version":"1.0","type":"link"}