{"product_id":"bek1065","title":"Mouse Galectin-1 ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of Galect in -1 in mouse serum, plasma, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Galectin-1 is a member of the galectins, which are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. In Mouses it is encoded by the LGALS1 gene,which maps to 22q12-q13. It is expressed by the endometrial stromal cells throughout the menstrual cycle, however significantly increases during implantation. The Galectin-a plays a role in the immunosuppression required for a successful pregnancy, andit may also act as an autocrine negative growth factor that regulates cell proliferation.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Galectin-1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-GALECTIN-1 polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Galectin-1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Galectin-1 can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 156 pg\/ml - 10,000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 5 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409019441432,"sku":"BEK1065","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_02195e66-002d-401c-bf14-43317634fb77.jpg?v=1750988368","url":"https:\/\/danabiosci.com\/products\/bek1065","provider":"Dana Bioscience","version":"1.0","type":"link"}