{"product_id":"bek1050","title":"Human Eotaxin ELISA Kit","description":"\u003cstrong\u003eApplication:\u003c\/strong\u003e For quantitative detection of eotax in in human serum, plasma, body fluids, tissue lysates or cell culture supernatants.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eIntroduction:\u003c\/strong\u003e Eotaxin, also known as Chemokine (C-C motif) ligand 11 (CCL11), is a small cytokine belonging to the CC chemokine family. These family members play an important role in the recruitment of leukocytes to inflammatory lesions. Eotaxin is a potent inducer of eosinophil chemotaxis leading to eosinophil migration in vitro and accumulation in vivo. It also interacts with CCR2 and CCR5 and can thus affect the responses of monocytes, which express both receptors. CCL11 selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergic responses. It has been found that eotaxin has high degree selectivity for its receptor, such that they are inactive on neutrophils and monocytes, which do not express CCR3.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003ePrinciple of the Assay:\u003c\/strong\u003e This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-eotaxin polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-eotaxin polyclonal antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the eotaxin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of eotaxin can be calculated.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eStorage and Expiration:\u003c\/strong\u003e Store at 2-8℃ for 6 months, or at -20℃ for 12 months.\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eRange:\u003c\/strong\u003e 15.6 pg\/ml - 1000 pg\/ml\u003cbr\u003e\u003cbr\u003e\u003cstrong\u003eSensitivity:\u003c\/strong\u003e \u0026lt; 5 pg\/ml","brand":"Biospes","offers":[{"title":"96T","offer_id":50409017934104,"sku":"BEK1050","price":500.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/files\/Biospes-logo_5ff599b8-8ee2-47ba-858b-c48ae69b0659.jpg?v=1750988332","url":"https:\/\/danabiosci.com\/products\/bek1050","provider":"Dana Bioscience","version":"1.0","type":"link"}