{"product_id":"anti-rwdd3-antibody-abcf07378","title":"anti- RWDD3 antibody","description":"\u003ch4\u003eHost\u003c\/h4\u003e\u003cp\u003eRabbit\u003c\/p\u003e\u003ch4\u003eUniprot ID\u003c\/h4\u003e\u003cp\u003eQ9Y3V2\u003c\/p\u003e\u003ch4\u003eForm\u003c\/h4\u003e\u003cp\u003eliquid\u003c\/p\u003e\u003ch4\u003ePurification\u003c\/h4\u003e\u003cp\u003eImmunogen affinity purified\u003c\/p\u003e\u003ch4\u003ePurity\u003c\/h4\u003e\u003cp\u003e \u003c\/p\u003e\u003cp\u003e\n\u0026gt;= 95%\u003c\/p\u003e as determined by SDS-PAGE\u003ch4\u003eClonality\u003c\/h4\u003e\u003cp\u003epolyclonal\u003c\/p\u003e\u003ch4\u003eClone ID\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eIsotype\u003c\/h4\u003e\u003cp\u003eIgG\u003c\/p\u003e\u003ch4\u003eStorage\u003c\/h4\u003e\u003cp\u003ePBS with 0.02% sodium azide and 50% glycerol pH 7.3,-20℃ for 12 months(Avoid repeated freeze \/ thaw cycles.)\u003c\/p\u003e\u003ch4\u003eBackground\u003c\/h4\u003e\u003cp\u003eEnhancer of SUMO conjugation. Via its interaction with UBE2I\/UBC9, increases SUMO conjugation to proteins by promoting the binding of E1 and E2 enzymes, thioester linkage between SUMO and UBE2I\/UBC9 and transfer of SUMO to specific target proteins which include HIF1A, PIAS, NFKBIA, NR3C1 and TOP1. Isoform 1 and isoform 2 positively regulate the NF-kappa-B signaling pathway by enhancing the sumoylation of NF-kappa-B inhibitor alpha(NFKBIA), promoting its stabilization which consequently leads to an increased inhibition of NF-kappa-B transcriptional activity. Isoform 1 and isoform 2 negatively regulate the hypoxia-inducible factor-1 alpha(HIF1A) signaling pathway by increasing the sumoylation of HIF1A, promoting its stabilization, transcriptional activity and the expression of its target gene VEGFA during hypoxia. Isoform 2 promotes the sumoylation and transcriptional activity of the glucocorticoid receptor NR3C1 and enhances the interaction of SUMO1 and NR3C1 with UBE2I\/UBC9. Has no effect on ubiquitination.\u003c\/p\u003e\u003ch4\u003eImmunogen\u003c\/h4\u003e\u003cp\u003eRWD domain containing 3\u003c\/p\u003e\u003ch4\u003eSynonyms\u003c\/h4\u003e\u003cp\u003eRSUME\u003c\/p\u003e\u003ch4\u003eObserved MW\u003c\/h4\u003e\u003cp\u003e\u003c\/p\u003e\u003ch4\u003eReactivity\u003c\/h4\u003e\u003cp\u003eHuman\u003c\/p\u003e\u003ch4\u003eTested Application\u003c\/h4\u003e\u003cp\u003eELISA, IHC\u003c\/p\u003e\u003ch4\u003eRecommended dilution\u003c\/h4\u003e\u003cp\u003eIHC: 1:20-1:200\u003c\/p\u003e\u003ch4\u003eGene ID\u003c\/h4\u003e\u003cp\u003e25950\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eCell Division and Proliferation\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"100µg \/ Rabbit \/ Q9Y3V2","offer_id":42542024261866,"sku":"ABCF07378","price":280.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/758-7580448_monoclonal-antibody-png-transparent-png_40d6f629-bd12-48ba-b753-d60dcd71b2c9.png?v=1646164358","url":"https:\/\/danabiosci.com\/products\/anti-rwdd3-antibody-abcf07378","provider":"Dana Bioscience","version":"1.0","type":"link"}