{"product_id":"abcc26614","title":"ELISA Kit for Protease Activated Receptor 1 (PAR1)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e0.156-10ng\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eHomo sapiens (Human)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 0.054ng\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eTissue homogenates, cell lysates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Protease Activated Receptor 1 (PAR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Protease Activated Receptor 1 (PAR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Protease Activated Receptor 1 (PAR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Protease Activated Receptor 1 (PAR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eFIIR; F2r; CF2R; HTR; PAR1; TR; Coagulation Factor II Receptor; Thrombin Receptor\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eProtease Activated Receptor 1\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eCardiovascular biology;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/24390664\"\u003eIs there any diagnostic value of serum protease-activated receptor-1 (PAR1) levels on determination of epithelial ovarian carcinoma?\u003c\/a\u003e;\u003ca href=\"https:\/\/www.researchgate.net\/publication\/304781507_Clinical_significance_of_serum_protease-activated_receptor_1_PAR1_level_in_patients_with_breast_cancer\"\u003eClinical significance of serum protease-activated receptor 1 (PAR1) level in patients with breast cancer\u003c\/a\u003e;\u003ca href=\"https:\/\/doi.org\/10.3892\/etm.2017.5662\"\u003eProteinase 3 expression on the neutrophils of patients with paroxysmal nocturnal hemoglobinuria\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 0.054ng\/mL \/ Homo sapiens (Human)","offer_id":42481916182762,"sku":"ABCC26614","price":700.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_59f13051-beb6-44c1-816d-f992342d0c00.png?v=1644868510","url":"https:\/\/danabiosci.com\/products\/abcc26614","provider":"Dana Bioscience","version":"1.0","type":"link"}