{"product_id":"abcc26574","title":"ELISA Kit for Apelin 13 (AP13)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e74.07-6000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eRattus norvegicus (Rat)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 26.38pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma and other biological fluids.\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e2h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Apelin 13 (AP13) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Apelin 13 (AP13) and unlabeled Apelin 13 (AP13) (Standards or samples) with the pre-coated antibody specific to Apelin 13 (AP13). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Apelin 13 (AP13) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Apelin 13 (AP13) in the sample.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eAPLN13\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eApelin 13\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eMetabolic pathway;Endocrinology;Cardiovascular biology;Neuro science;Gastroenterology;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"http:\/\/apjcpcontrol.net\/paper_file\/issue_abs\/Volume13_No8\/4025-30%207.27%20Javad%20Ashrafi.pdf\"\u003eIs short-term exercise a therapeutic tool for improvement of cardioprotection against DOX-induced cardiotoxicity? An experimental controlled protocol in rats.\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/22578103\"\u003eIndividual and concomitant effects of cardioprotective programs on cardiac apelinergic system and oxidative state in L-NAME-induced hypertension.\u003c\/a\u003e;\u003ca href=\"http:\/\/www.zjrms.ir\/browse.php?a_code=A-10-2029-1\u0026amp;slc_lang=en\u0026amp;sid=1\"\u003eImprovement of Kidney Apelin and Apelin Receptor in Nitro-L-Arginine-Methyl Ester Induced Rats\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 26.38pg\/mL \/ Rattus norvegicus (Rat)","offer_id":42481914183914,"sku":"ABCC26574","price":722.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_1d300647-6f4b-4f03-a03f-5e6bb8519fd6.png?v=1644868441","url":"https:\/\/danabiosci.com\/products\/abcc26574","provider":"Dana Bioscience","version":"1.0","type":"link"}