{"product_id":"abcc26396","title":"ELISA Kit for Programmed Cell Death Protein 1 (PD1)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e0.156-10ng\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eHomo sapiens (Human)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 0.063ng\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eTissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Programmed Cell Death Protein 1 (PD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Programmed Cell Death Protein 1 (PD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Programmed Cell Death Protein 1 (PD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Programmed Cell Death Protein 1 (PD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eCD279; PDCD1; SLEB2; HPD1P\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eProgrammed Cell Death Protein 1\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eApoptosis;Immune molecule;Endocrinology;Autoimmunity;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28545019\"\u003eCirculating soluble programmed death-1 levels may differentiate immune-tolerant phase from other phases and hepatocellular carcinoma from other clinical diseases in chronic hepatitis B virus infection.\u003c\/a\u003e;\u003ca href=\"\"\u003eПотенциальные биомаркеры эффективности терапии ниволумабом при метастатическом почечно-клеточном раке\u003c\/a\u003e;\u003ca href=\"https:\/\/www.malignanttumors.org\/jour\/article\/view\/560\"\u003eАНАЛИЗ РЕЗУЛЬТАТОВ ЛЕЧЕНИЯ БОЛЬНЫХ МЕТАСТАТИЧЕСКИМ РАКОМ ПОЧКИ, ПОЛУЧАВШИХ АНТИ-PD-1‑ТЕРАПИЮ В РАМКАХ ПРОГРАММЫ …\u003c\/a\u003e;\u003ca href=\"https:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0212978\"\u003eClinical significance of soluble programmed cell death-1 and soluble programmed cell death-ligand 1 in patients with locally advanced rectal cancer treated …\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 0.063ng\/mL \/ Homo sapiens (Human)","offer_id":42481894949098,"sku":"ABCC26396","price":630.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_9210bee6-b248-40b1-8c0f-e806d7808483.png?v=1644868168","url":"https:\/\/danabiosci.com\/products\/abcc26396","provider":"Dana Bioscience","version":"1.0","type":"link"}