{"product_id":"abcc26066","title":"ELISA Kit for Heat Shock Protein 70 (HSP70)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e3.12-200ng\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eHomo sapiens (Human)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 1.33ng\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e3h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eDouble-antibody Sandwich\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock Protein 70 (HSP70). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock Protein 70 (HSP70). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock Protein 70 (HSP70), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Heat Shock Protein 70 (HSP70) in the samples is then determined by comparing the O.D. of the samples to the standard curve.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eDnaK; 70 Kilodalton Heat Shock Proteins\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eHeat Shock Protein 70\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eTumor immunity;Infection immunity;Developmental science;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"http:\/\/www.springerlink.com\/content\/b8738j3571287425\/\"\u003eProteomic analysis reveals significant elevation of heat shock protein 70 in patients with chronic heart failure due to arrhythmogenic right ventricular cardiomyopathy\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC3549857\/\"\u003eNeuroinflammation in autism spectrum disorders\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/23455589\"\u003eLuteal serum BDNF and HSP70 levels in women with premenstrual dysphoric disorder\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26055465\"\u003eConcentraciones de proteínas de estrés térmico, L-arginina y dimetilarginina asimétrica en pacientes con síndrome de apnea obstructiva del sue\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/%2026055465\"\u003eHeat Shock Proteins,\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/26518955\"\u003eL-Alanyl-Glutamine Attenuates Oxidative Stress in Liver Transplantation Patients\u003c\/a\u003e;\u003ca href=\"http:\/\/www.ejmanager.com\/mnstemps\/33\/33-1429777310.pdf\"\u003eElevated interictal serum HSP-70 levels as an indicator of neurodegeneration for chronic migraine\u003c\/a\u003e;\u003ca href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0144860916301935\"\u003ePhysiological consequences for rainbow trout (Oncorhynchus mykiss) of short-term exposure to increased suspended solid load\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28531762\"\u003eHigh-dose testosterone enanthate supplementation boosts oxidative stress, but exerts little effect on the antioxidant barrier in sedentary adolescent male rat liver.\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28122311\"\u003eSIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death.\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29111316\"\u003eConcentrations of SP-A and HSP70 are associated with polarization of macrophages in pleural effusions of non-small cell lung cancer\u003c\/a\u003e;\u003ca href=\"http:\/\/search.ebscohost.com\/login.aspx?direct=true\u0026amp;profile=ehost\u0026amp;scope=site\u0026amp;authtype=crawler\u0026amp;jrnl=20900570\u0026amp;AN=125788660\u0026amp;h=IheeS7P6RfHRjJUvOdUzqedghaePp0SpSxletD%2BkQYas7IGdzf0W3k6CdUoUm0pmm%2BF7Hk3%2FLwmqvB94JjBd0A%3D%3D\u0026amp;crl=c\"\u003eEFFECT OF DIETARY SOURCE OF SELENIUM ON PRODUCTIVE AND REPRODUCTIVE PERFORMANCE OF SINAI LAYING HENS UNDER HEAT STRESS CONDITIONS.\u003c\/a\u003e;\u003ca href=\"http:\/\/www.sciencedirect.com\/science\/article\/pii\/S0753332216328098\"\u003eSIRT1 inhibits releases of HMGB1 and HSP70 from human umbilical vein endothelial cells caused by IL-6 and the serum from a preeclampsia patient and protects the cells from death\u003c\/a\u003e;\u003ca href=\"https:\/\/www.sciencedirect.com\/science\/article\/pii\/S004484861731548X\"\u003ePhysiological consequences of chronic exposure of rainbow trout (Oncorhynchus mykiss) to suspended solid load in recirculating aquaculture systems\u003c\/a\u003e;\u003ca href=\"https:\/\/www.fasebj.org\/doi\/abs\/10.1096\/fj.201800673RR\"\u003eSignature-oriented investigation of the efficacy of multicomponent drugs against heart failure\u003c\/a\u003e;\u003ca href=\"https:\/\/scialert.net\/fulltext\/?doi=jbs.2019.161.172\"\u003eMaged Mohamed Abd-Elhakim Barakat, Noha Ahmed El-Boghdady, Ebtehal Kamal Eldin Farrag, Ataa Abdelhalim Said and Sylvia Edward Shaker\u003c\/a\u003e;\u003ca href=\"https:\/\/scialert.net\/fulltextmobile\/?doi=jbs.2019.161.172\"\u003eProtective and Curative Effects of Bombax ceiba Flower and Ziziphus spina christi Fruit Extracts on Gastric Ulcer\u003c\/a\u003e;\u003ca href=\"http:\/\/researchonline.ljmu.ac.uk\/id\/eprint\/10638\/\"\u003ePHYSIOLOGICAL ADAPTATIONS TO HEAT ACCLIMATION; REPERCUSSIONS ON CYCLING PERFORMANCE\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 1.33ng\/mL \/ Homo sapiens (Human)","offer_id":42481869095146,"sku":"ABCC26066","price":630.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_b685c985-2646-4106-bf0f-aad11e828d2a.png?v=1644867666","url":"https:\/\/danabiosci.com\/products\/abcc26066","provider":"Dana Bioscience","version":"1.0","type":"link"}