{"product_id":"abcc25653","title":"ELISA Kit for Corticotropin Releasing Factor (CRF)","description":"\u003ch4\u003eFormat\u003c\/h4\u003e\u003cp\u003e48T, \u003ci\u003e96T\u003c\/i\u003e, 96T×5, 96T×10, 96T×100\u003c\/p\u003e\u003ch4\u003eDetection range\u003c\/h4\u003e\u003cp\u003e12.35-1,000pg\/mL\u003c\/p\u003e\u003ch4\u003eApplication\u003c\/h4\u003e\u003cp\u003eEnzyme-linked immunosorbent assay for Antigen Detection.\u003c\/p\u003e\u003ch4\u003eOrganism species\u003c\/h4\u003e\u003cp\u003eMus musculus (Mouse)\u003c\/p\u003e\u003ch4\u003eSensitivity\u003c\/h4\u003e\u003cp\u003eThe minimum detectable dose of this kit is typically less than 5.05pg\/mL\u003c\/p\u003e\u003ch4\u003eSample type\u003c\/h4\u003e\u003cp\u003eSerum, plasma and other biological fluids\u003c\/p\u003e\u003ch4\u003eAssay length\u003c\/h4\u003e\u003cp\u003e2h\u003c\/p\u003e\u003ch4\u003eMethod\u003c\/h4\u003e\u003cp\u003eCompetitive Inhibition\u003c\/p\u003e\u003ch4\u003eTest principle\u003c\/h4\u003e\u003cp\u003eThis assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Corticotropin Releasing Factor (CRF) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Corticotropin Releasing Factor (CRF) and unlabeled Corticotropin Releasing Factor (CRF) (Standards or samples) with the pre-coated antibody specific to Corticotropin Releasing Factor (CRF). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Corticotropin Releasing Factor (CRF) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Corticotropin Releasing Factor (CRF) in the sample.\u003c\/p\u003e\u003ch4\u003eAlternative Names\u003c\/h4\u003e\u003cp\u003eCRH; Corticotropin Releasing Hormone; Corticoliberin\u003c\/p\u003e\u003ch4\u003eItem Name\u003c\/h4\u003e\u003cp\u003eCorticotropin Releasing Factor\u003c\/p\u003e\u003ch4\u003eResearch Area\u003c\/h4\u003e\u003cp\u003eEndocrinology;Cardiovascular biology;Neuro science;Hormone metabolism;\u003c\/p\u003e\u003ch4\u003eReference\u003c\/h4\u003e\u003cp\u003e\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/30038568\"\u003eVaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary …\u003c\/a\u003e;\u003ca href=\"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/29379286\"\u003eThe depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic …\u003c\/a\u003e;\u003ca href=\"https:\/\/www.fasebj.org\/doi\/abs\/10.1096\/fj.201900100R\"\u003eContribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in mice\u003c\/a\u003e;\u003c\/p\u003e","brand":"ABCbiolab","offers":[{"title":"96T \/ The minimum detectable dose of this kit is typically less than 5.05pg\/mL \/ Mus musculus (Mouse)","offer_id":42481824792810,"sku":"ABCC25653","price":648.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0590\/5652\/1400\/products\/74120318_padded_logo_f532f87d-e544-40fd-8d7e-726757703baf.png?v=1644866942","url":"https:\/\/danabiosci.com\/products\/abcc25653","provider":"Dana Bioscience","version":"1.0","type":"link"}