Unlock the Secrets of "Organoid"

Organoids are in vitro generated three-dimensional (3D), mini cluster of cells that highly simulate the structure and function of the corresponding organ in vivo. In 2009, Hans Clevers and colleagues first demonstrated that single Lgr5+-expressing intestinal adult stem cells (ASCs) could self-organize and differentiate to form intestinal crypt-villus structures that contained all intestinal cell types. This ushered in a new era in the development of organoid technology. In the following decades, organoids were constructed and employed as a novel research model that could be used instead of traditional cell lines and heterogeneous animal models (Click to Know More "Organoids").

Since the successful establishment of epithelial organoids in 2009, organoid cultures have been used in various organs. CUSABIO presents the Critical Factors in various organoid cultures (Brain Organoids; Lung Organoids ; Liver Organoids ; Kidney Organoids ; Intestinal Organoids ; Gastric Organoids etc.) and Targets related disease pathology to support Organoid In Vitro Model Research!

● The Critical Factors in "Organoid Cultures"

Organoids	The Critical Factors in "Organoid Cultures"
Gastric Organoids	EGF FGF-10 Noggin R-spondin 1 Wnt-3a
Small intestine and colonic epithelial Organoids	EGF Noggin R-spondin 1 Wnt-3a
Liver Organoids	BMP-4 EGF bFGF FGF-10 HGF R-spondin 1 Wnt-3a R-spondin 1 Wnt-3a
Pulmonary Organoids	Activin A bFGF FGF-4 Noggin
Prostate Organoids	Activin A EGF bFGF FGF-10 Noggin R-spondin 1 Wnt-10b
Pancreatic Organoids	EGF FGF-10 Noggin R-spondin 1 Wnt-10b Wnt-3a
Brain Organoids	bFGF Noggin DKK1 EGF BDNF GDNF
Inner Ear Organoids	BMP-4 bFGF
Retinal Organoids	Shh Wnt-3a
Breast Organoids	Noggin R-spondin 1 R-spondin 2 EGF bFGF FGF-10 Wnt-3a Prolactin
Kidney Organoids	BMP-2 BMP-4 BMP-7 bFGF FGF-9
Cardiac Organoids	bFGF FGF-4 FGF-10 LIF

● The Targets in "Organoid" In Vitro Model Research

ACE2	AHR	AXL	APC	CAT	CD44	CXCR2	CFTR
CLOCK	CRB1	CHD8	DCLK1	EPCAM	EGF	IL22	Gastrin
KRAS	LGR5	MET	MYC	MTOR	NOD2	OAT	PTEN
PIGS	PG-E2	RB1	RHO	RHOA	RNF43	RIPK1	Retinoic acid
STAT3	SOX2	SOX9	SPOP	SIRT1	TAZ	Testosterone	TLR2
TMPRSS2