One-step method EasyStep principle
The kit adopts the sandwich method principle. Coat the specific antibody in a 96-well microplate, add the standard/sample, so that the protein in the standard or the protein in the sample binds to the antibody solid-phased on the microplate, and then add the HRP-coupled antibody. , after thoroughly washing the unbound HRP-conjugated antibody, add TMB substrate to develop color. TMB is converted into blue under the catalysis of peroxidase and into the final yellow under the action of acid. The depth of color is positively correlated with the target protein in the sample. Use a microplate reader to measure the absorbance (OD value) at a wavelength of 450 nm, and calculate the sample concentration by drawing a standard curve.