Why Use Glycerol-Free Enzymes
for PCR and LAMP Reagent Development?
Glycerol is commonly used to stabilize molecular biology enzymes. For lyophilized PCR reagents, custom master mixes, and molecular diagnostic kit development, however, a glycerol-free formulation may provide greater control over the final reagent composition.
Many DNA polymerases, reverse transcriptases, and other molecular biology enzymes are supplied in buffers containing glycerol.
This is generally suitable for routine PCR workflows. But when developing a commercial master mix or lyophilized molecular diagnostic reagent, the formulation of the raw enzyme itself becomes an important consideration.
Where Are Glycerol-Free Enzymes Useful?
Greater control over excipients and the final freeze-drying formulation.
Allows developers to optimize glycerol concentration as part of the complete formulation.
Useful for PCR and RT-qPCR reagents designed for simplified storage and handling.
Glycerol-free Bst polymerase and reverse transcriptase options are available for isothermal amplification.
Available Molecular Biology Raw Materials
| Product Type | Typical Application |
|---|---|
| Glycerol-Free Hot-start Taq DNA Polymerase | PCR / qPCR |
| Glycerol-Free M-MLV Reverse Transcriptase | RT-PCR / RT-qPCR |
| Glycerol-Free RNase Inhibitor | RNA workflows |
| Glycerol-Free Heat-Labile UDG | Carryover contamination control |
| Glycerol-Free Bst DNA Polymerase | LAMP |
| Glycerol-Free RTL Reverse Transcriptase | RT-LAMP |
From Raw Enzymes to Lyophilized Master Mixes
In addition to individual enzymes, molecular diagnostic reagent options include Liquid Lyoph-Ready, Freeze-Dryable Liquid, Lyophilized Beads, and In Situ Lyophilization formats.
PCR and LAMP development support can also include primer and probe optimization, reagent performance optimization, lyophilization process development, CDMO, OEM, and custom kit development.
Looking for PCR or LAMP Raw Materials?
Dana Bioscience can help identify glycerol-free enzymes, Hot-start Taq polymerases, reverse transcriptases, RNase inhibitors, UDG, Bst polymerases, dNTPs, and PCR/LAMP master mixes for your molecular biology or assay development project.
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