Viral vector production is a core part of gene therapy research. Both AAV and lentiviral systems require a structured workflow that includes production, purification, quantification, and quality assessment.
Typical Production Steps
- Plasmid preparation
- Cell transfection
- Vector harvest
- Purification
- Titer measurement
- Quality assessment
Important Analytical Readouts
- AAV titer assays
- Lentivirus quantification
- Residual DNA testing
- Host Cell Protein analysis
A standardized production workflow helps improve batch consistency and data reproducibility.