Cell therapy manufacturing involves multiple steps, from cell sourcing and expansion to characterization, contamination testing, and functional assessment. Even at the research stage, understanding a manufacturing-oriented workflow can improve development decisions.
Main Workflow Steps
- Cell isolation or thawing
- Cell activation and expansion
- Cell counting and viability assessment
- Phenotype analysis
- Mycoplasma detection
- Functional testing
Important Analytical Readouts
- Cell counting
- Flow cytometry
- Mycoplasma detection
- Cytokine ELISA
A structured workflow makes it easier to compare culture conditions, monitor cell quality, and support process development.