Splenocytes in Immunological Research: Across Species and Isolation, Freezing, Thawing Steps

Splenocytes in Immunological Research: Across Species and Isolation, Freezing, Thawing Steps

Keywords: Splenocytes (SPLs); Cynomolgus Monkey Splenocytes; Rhesus Monkey Splenocytes; Dog Splenocytes; Canine Splenocytes; Rat Splenocytes; Mouse Splenocytes; Mice Splenocytes; Rabbit Splenocytes; Isolation; Freezing; Thawing

📦 IPHASE Splenocyte Product Lineup

Product Name Specification
IPHASE Human Spleen Mononuclear Cells Isolation Kit 1 Kit
IPHASE Monkey Spleen Mononuclear Cells Isolation Kit 1 Kit
IPHASE Dog (Beagle) Spleen Mononuclear Cells Isolation Kit 1 Kit
IPHASE Rat Spleen Mononuclear Cells Isolation Kit 1 Kit
IPHASE Mouse Spleen Mononuclear Cells Isolation Kit 1 Kit
IPHASE Rabbit Spleen Mononuclear Cells Isolation Kit 1 Kit
IPHASE Monkey (Cynomolgus) SMC, Frozen 5 million
IPHASE Dog (Beagle) SMC, Frozen 5 million
IPHASE Mouse (ICR/CD-1) SMC, Frozen 5 million
IPHASE Mouse (C57BL/6) SMC, Frozen 5 million
IPHASE Mouse (C57BL/6) CD4+ T Cells, Frozen 1 million
IPHASE Mouse (C57BL/6) CD8+ T Cells, Frozen 1 million
IPHASE Mouse (BALB/c) CD4+ T Cells, Frozen 1 million
IPHASE Mouse (BALB/c) CD8+ T Cells, Frozen 0.5 million
IPHASE Rabbit (New Zealand White) SMC, Fresh 5 million

🧠 What Are Splenocytes (SPLs)?

Splenocytes are a diverse group of immune cells isolated from the spleen, including T cells, B cells, macrophages, dendritic cells, and NK cells. These cells are essential for both adaptive and innate immune responses and are widely used in preclinical and translational research.

🔬 Role and Composition

The spleen contains red pulp (blood filtration) and white pulp (immune response initiation). The white pulp’s structure, including T and B cell zones, makes SPLs ideal for immunological assays such as ELISPOT, flow cytometry, and MLR (mixed lymphocyte reactions).

🧬 Species-Specific Applications

  • Cynomolgus Monkey: Preferred for translational studies due to human-like immune response.
  • Rhesus Monkey: Used to bridge preclinical and clinical research.
  • Dog (Canine): Applied in veterinary immunology and comparative studies.
  • Rabbit: Common in vaccine development and antibody research.
  • Mouse (Mice): Most commonly used; ideal for standardized immunology protocols.
  • Rat: Used in toxicology and complementary to murine models.

🧫 Splenocyte Isolation Protocol

Splenocytes are isolated under aseptic conditions. The spleen is processed in RPMI 1640 medium, followed by centrifugation and washing. Leukocyte layers are extracted and washed 1–2 times before use in assays.

❄️ Freezing Splenocytes

Cells are suspended in cryopreservation medium, aliquoted, and frozen at -80°C. For long-term storage, samples are transferred to -150°C or liquid nitrogen. Proper freezing preserves functionality and viability.

🔥 Thawing Splenocytes

Thawing is performed quickly at 37°C to minimize DMSO toxicity. Cells are gently resuspended, washed, and incubated in a CO2 incubator for 1 hour. Viability is confirmed by counting active cells post-resuspension.

✅ Conclusion

Splenocytes are indispensable in modern immunology research. Whether from rodents, primates, rabbits, or canines, their isolation, freezing, and thawing are crucial for reproducible and reliable results in immune system studies. As immunological understanding advances, SPLs will remain foundational to both human and veterinary medicine research.

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