Mycoplasma contamination is one of the most common problems in cell culture laboratories. Reliable mycoplasma detection is essential to ensure experimental integrity.
Why Mycoplasma Is a Serious Problem
- Alters gene expression
- Affects cell growth
- Changes metabolic activity
Common Detection Methods
- PCR-based detection
- DNA staining
- Microbial culture
PCR-Based Detection
PCR-based mycoplasma detection kits are widely used due to their high sensitivity and ability to detect contamination quickly.