Mycoplasma contamination is one of the most common problems in cell culture research. It can affect cell growth, metabolism, and gene expression, leading to unreliable data.
Common Causes
- Introduction of contaminated cell lines
- Cross-contamination from personnel or equipment
- Use of contaminated reagents
Prevention Strategies
- Routine mycoplasma detection testing
- Screening newly acquired cell lines
- Strong aseptic technique
- Careful reagent quality control
Conclusion
Prevention is the best defense against mycoplasma contamination, and PCR-based mycoplasma detection remains one of the most effective monitoring strategies.