Lentiviral vectors are widely used in gene delivery, CRISPR screening, and cell engineering applications. Accurate lentivirus quantification helps researchers control multiplicity of infection (MOI) and optimize transduction conditions.
Why Lentivirus Titer Matters
The viral titer determines how many cells receive the genetic payload. Incorrect titration can lead to poor gene delivery efficiency or excessive toxicity.
Common Lentivirus Quantification Methods
- p24 ELISA assays
- qPCR-based viral genome quantification
- Functional transduction assays
p24 ELISA Assays
p24 ELISA detects the capsid protein of lentiviral particles and is commonly used for rapid viral particle quantification.
Functional Titer Assays
Functional assays measure the number of infectious particles capable of delivering genes to target cells. Although more labor-intensive, this method provides biologically relevant results.