AAV (Adeno-Associated Virus) vectors are widely used in gene therapy research. Accurate AAV titer measurement is essential for evaluating vector production, transduction efficiency, and experimental reproducibility.
Why AAV Titer Matters
Vector concentration directly influences gene delivery performance. Reliable titer determination allows researchers to compare vector batches, optimize production workflows, and improve experimental consistency.
Common AAV Titer Measurement Methods
- qPCR-based genome quantification
- Digital PCR (dPCR)
- ELISA-based capsid quantification
- Cell-based functional assays
ELISA-Based AAV Titer Assays
ELISA assays detect viral capsid proteins and provide a rapid and reproducible approach to estimate vector concentration. Many laboratories use ELISA for routine viral vector analysis.